I want to determine the bacterial load in different organs in mice model during the bacterial infection and after its treatment by IHC and immunofluorescence method.
Thank you very much Susan for valuable link
Most of us can list organizations known for not only their economic success but their successes in building a culture in which workers are proud and happy to belong. In healthy-workplace job the...
02 March 2019 4,660 1 View
I am interested to find out the effect of a drug in alteration of NMDAR and glucocorticoid receptor (GR) levels in mice model by IHC using FITC tagged antibody. I am gating non specific (may be...
07 August 2014 710 6 View
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31 December 2011 9,233 2 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.
14 July 2024 485 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
Immunofluorescence staining of mouse spleen paraffin sections showed strong fluorescence signals in red blood cells in the control section without antibodies. Is it autofluorescence of red blood...
02 July 2024 827 3 View
I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...
28 June 2024 353 1 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View