Is there an openware/ freeware to predict solubility and stability of a molecule in multiple solvent?

Hello, I am trying to determine solubility of a well known molecule in multiple solubilizers. Is there an openware where I can upload the structures of both (drug substance and solvent) and get a...

09 October 2019 5,720 2 View

How do you find odorant binding protein in a genome?

I am working on Black fly. I just received the genome fasta file. I want to find the odorant binding protein in the genome. Which software should I use? And who should I consider as my reference...

06 July 2014 7,546 9 View

How does one decide which nuclear hormone receptors are relevant for human pathogenicity? What genomic resources should I use?

In brugia malayi there are many NHR encoded in the genome. How does one pick the relevant one that cause host pathogenicity?

06 July 2014 9,376 1 View

How does one determine therapeutic potential of a nuclear hormone receptor?

When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...

06 July 2014 5,145 1 View

How to identify operons using transcriptomic data?

Nematodes have unique genomic features for eukaryotes, such as the existence of operons. These features require special analytical methods in genomics. Suppose you are analyzing a transcriptome...

06 July 2014 2,651 5 View

Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...

04 May 2014 9,858 4 View

Should I consider a spliced version of the gene to build the qPCR primers or an unspliced version which has an intron?

I ran an RNAseq with Brugia malayi worms treated with a drug and untreated. I found a certain number of genes differentially expressed in the treated worms as compared to the untreated. I selected...

03 April 2014 764 7 View

How can FPKM and RPKM be compared?

I have two samples, one is drug-treated and the other is not. I ran MiSeq on my samples and generated FPKM values. Some other author had done similar studies earlier and I want to compare my FPKM...

02 March 2014 4,457 3 View

His tagged protein not binding to Ni-NTA coulmn.

I am expressing a 6xHis tagged protein at C-terminal. I can see it in the supernatent,washes and flowthrough . But it does not stick to the nickel column. I use PBS+10%glycerol as my buffer. I...

07 August 2013 3,685 22 View

Should I refold my his-tagged protein after Triton-X100 digestion?

I am working on ligand binding domains and I am expressing the protein in the yeast system. I have solubilized my protein using Triton-X100. I do know that it is a non-ionic detergent and it...

07 August 2013 8,359 17 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View