Specifically, there are two main types of settings for mice: 2mA, 2s and 0.3 mA, 5 s. What is the difference? Which is more sensitive? How Passive Avoidance test results may be different between out bread white mice and C57Bl/J mice?
Please learn about the experience of other scholars who compared the features of behavioral tests in mice of different lines (as compared with rats). All the best.
Consider using either step -through or step -down latency test for one trial passive avoidance test. Step-through would be better.
Here you can find a detailed protocol for step-through one-trial passive avoidance in mice:
https://www.researchgate.net/publication/259956779_Behavioral_Methods_for_the_Study_of_the_Ras-ERK_Pathway_in_Memory_Formation_and_Consolidation_Passive_Avoidance_and_Novel_Object_Recognition_Tests
Article Behavioral Methods for the Study of the Ras–ERK Pathway in M...