Can anybody recommend a good antibody for westerns of p110-alpha in human cells?

19 November 2014 1 4K Report

We have tried several commercially available antibodies to p110 alpha in NETN extracts from HeLa cells but they do not seem to be picking up a band of the expected size. Can anyone help? thanks

Konstantin Yakimchuk

Hello,

I recommend an antibody database available at labome.com. For p110-alpha antibodies for Western blot, you can check this link: https://www.labome.com/review/gene/human/p110-antibody.html. Santa Cruz Biotechnology has two anti- p110-alpha abs used in western blot on human samples: rabbit polyclonal (H-201) sc-7174 (dilution - 1:500), goat polyclonal (C-17) sc-1331 (dilution - 1:500) (ref: Salm et al., PLoS One. 2015). Also, ABcam provides rabbit monoclonal (EP383Y) ab40776 (ref: Xu et al., J Proteomics 2015). Cell Signaling Technology has rabbit monoclonal (C73F8) p110 antibody (Cell signaling, 4249) (ref: Kitatani et al., Oncogene 2015). Also, EMD Millipore offers rabbit polyclonal p110 antibody (Millipore, 09-481) (ref: Luo et al., Int J Biochem Cell Biol. 2013).

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