Please find the table of ammonium sulphate precipitation provided as appendix in the attached document

What you mean by Half saturation and full saturation? Most of the proteins get saturated at 70-80% (NH4)2SO4. The wt can be determined from the pptn table that Sumit had provided. For 100 ml culture, if you want to do 80 % saturation then the amount of NH4)2SO4 will be approx. 56 g. IYou can start with 25%, 50% and 75% saturation. then estimate the protein content.

@ Sumit Kumar, Thanks a lot for your valuable answer with suitable reference.

@ Rakesh, Half saturation and full saturation tests are used for identification of Albumin and Globulin in given unknown sample at Lab scale. Generally, only globulin can PPT out at Half saturation but Albumin don't PPT out at Half saturation, it requires full saturation for PPT out. Thanks for your Conclusive answer regarding Gms required for full saturation.

The other name of this Procedure is Salting out

the principle of this separation are

Lower the molecular weight of protein- Higher the salt required

higher the molecular weight of protein- low salt required 

Globulin (half saturation)

Albumin (Full saturation)