Can anyone share their Ideas about Pharmacological Research Work without Animal Use?

I am a Pharmacologist - Academician and would like to know and work on some Pharmacological research work which I can do at College level without use of any animal model. I mean to say some...

02 March 2016 5,566 2 View

Can anybody provide me with the best reference for how to perform half saturation test and full saturation test for protein?

07 August 2014 2,484 4 View

What could be a better alternative of experiments on isolated tissues of an animal (rat)? Are there any software to be used for a demonstration?

If we want to demonstrate the experiments on isolated rat ileum or any other isolated tissue, then do anybody have better alternate of this without dissecting a rat? If any software is available...

07 August 2014 140 1 View

Can anybody suggest any free download website for animated 3D screensaver?

I want to add animated 3D screensaver in Windows 8. Can anybody suggest any official and free website for this?

04 May 2014 4,441 0 View

Free software to conduct an online quiz?

From the last few months, I am trying to generate an online Quiz for my student, I have prepared some of them also, but find two difficulties: 1. Some students can't open the exam in their...

11 December 2013 1,466 4 View

What does the pD2 value of agonist indicate? Affinity or efficacy?

Up to my knowledge, pD2 is a marker of affinity of drug towards specific receptor and thus used to describe potency of medicine, but I still have a little confusion about it.

09 October 2013 1,710 4 View

How to measure cAMP level by simple laboratory technique or by diagnostic Kit?

I am checking cardiotonic activity & to perform in vitro study I need to measure cAMP level, for that I am searching for simple techniques which can be performed at laboratory level or best Kit...

09 October 2013 6,002 4 View

Shortcut key to merge some cells in table & to split the same!

Can anybody suggest the shortcut keys for Merging & splitting the cell in MS word & MS Excel?

09 October 2013 1,324 14 View

Can anybody suggest all the steps of fermentation processes required to synthesis Cynocobalamine?

If possible, give the detailed steps of fermentation process for Cynocobalamine with suitable reference.

08 September 2013 605 1 View

Which biomarkers can be useful to evaluate sedative & hypnotic activity?

I have evaluated Sedative, CNS-depressant, SKM relaxant as well as anxiolytic activity of one herb. To check sedative effect, I used Phenobarbital induce sleep model. Now, I would like know that...

07 August 2013 9,477 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View