How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Do anyone have full MATLAB code for Genetic Algorithm to solve objective function? And Also for the Economic load dispatch problem?

I need full matlab code of genetic algorithm to solve objective function.and also I need for Economic load dispatch problem.

22 December 2020 836 4 View

Variability during successive rounds on random oligonucleotide library synthesis?

I have a template for random oligonucleotide library synthesis, which will go successive 10-15 rounds of PCR before starting SELEX. Tell me if I am correct, the end product of one round will be...

19 November 2020 1,684 2 View

How we can decide the Gc column maximum temperature as manufactures providing 265/300 ?

I want to have one analysis on CP volamine GC column and i am getting some impurities are eluting at near about 280 degree temp up to 10 minute holding time. The column Box mentioned the temp....

18 November 2020 9,064 3 View

What will the effect on the inverse of large sparse (symmetric) matrix when a single (or a few) entries in the original matrix are perturbed?

The mathematics behind the inverse of large sparse matrices is very interesting and widely used in several fields. Sometimes, It is required to find the inverse of these kinds of matrices....

07 November 2020 4,842 3 View

NAND flash recovery?

Hi Due to not being able to access University tools etc. I am stuck using recovery software such as recuva etc. I guess the question I'm trying to ask is how would I go about analysing results...

12 October 2020 437 1 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

No title

Due to COVID- 19, pupil teacher of teacher training programs are doing school internship in online mode which they are completely opposed to. What do you think as to it will adversely affect their...

09 August 2020 756 20 View

What are the good sources or research papers to start studying GAN and its applications?

GAN is famous these days in the area of image processing for generating the unsupervised data. What can be the good sources to start studying the GAN and its applications.

25 May 2020 6,915 4 View

How to correct invalid atom type error in gromacs?

27 April 2020 1,318 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View