Rani Soni

4 Questions 7 Answers 0 Followers

Questions related from Rani Soni

How can I purify a protein cloned in pETm30 vector PI is 8.05? Why with both His tag and GST tag it is coming in flowthrough and wash fraction?

Only a small amount of protein is comming in soluble fraction even at 18degree with 0.3mM IPTG. On purifiying with both Ni NTA and glutathion beads it is coming out in flow through and wash...

09 March 2018 2,976 2 View

Can anybody please help me in that from where I can get genomic DNA of Plasmodium falciparum 3d7 strain?

3D7 strain of Plasmodium falciparum.

08 May 2016 4,080 6 View

How can I model an uncharacterized protein for docking but non homologous has been found against p blast ?

I want to use model for docking. Would it be possible to model it through modeller. Or please tell me about some threading based method.

05 January 2015 8,073 6 View

How can we pick DNA fragment if smear is formed in gel after restriction digetion?

Do we go for southern blotting?

01 June 2012 1,323 3 View