I am using SOX1 and Nestin as primary antibodies (1:200 and 1:500 for each) and Anti Goat Alex Fluor-594 and Anti Mouse Alexa555 as secondary antibodies (1:1000) and still not getting any results. Please help me with the concentration of primary and secondary antibodies or any other suggestions if I am doing something wrong. Thank you!
First, you need to try the source of the primary antibody (rabbit or mouse) and whether it matches the secondary antibody, as well as consult the instructions provided by the manufacturer to see if it's suitable for IF.
Additionally, it's important to avoid light during the experimental process.
If you can provide more information about the primary and secondary antibodies (such as Cat. and manufacturer ), I may be able to further assist you.
Hi, thank you for your response! I have used S Nestin (Anti-Human Nestin Antibody, Clone 10C2; Cat#60091 from Thermofisher Scientific)and SOX1 (Human SOX1 Affinity Purified Polyclonal Ab (100 UG),(Product Class: Human Antibodies from R&D Systems) and secondary antibodies from Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 and Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555.
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue