This is a blood smear done to a post mortem frog after few hours in -2C temperature for an experiment. how can the changes in erythrocyte morphology can be explained?
Valbona Aliko did you get the peripheral blood at post mortem or you collected the blood before its death and stored at -2C temperature? How did you obtain the blood you used for the smear?
I did the smear post mortem. We got it decapitating the frog. Since it wad dead we decided to thow out, but at the last moment I decided to do a blood smear.
Dear Valbona Aliko
As far as I understood correctly from the description of this case. We see an erythrocyte that has frozen - it has a white lumen in its cell, the ice has violated the integrity of the cell. And normal red blood cells that did not have time to freeze.
Regards, Sergey
Thank you all for your responses. It comes out from experiment that this species, P.kurtmuelleri/ridibundus did not tolerate freezing. So your interpretations fit perfectly with this idea.
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