Can any one tell me how to design primer for Methylation specific PCR?

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Vahhab Piranfar

please see this rules: ( maybe usefu)

http://www.urogene.org/methprimer/rules.html

or I'm trying with this PDF:

Methyl Primer Express® Getting Started Guide:

docs.appliedbiosystems.com/pebiodocs/04370961.pdf‎

Madhuram Khandelwal

Thanks. vahhab ...can you tell one more thing during designing of primer which part of promoter we have to choose .....as i thought i am selecting near start site is it right??