Madhuram Khandelwal

5 Questions 8 Answers 0 Followers

Questions related from Madhuram Khandelwal

Problem in trypsinization of cell line

I am working on Bladder cancer cell line that is HT 1376. I am having problem in trypsinization. For trypsinization after removing media from flask, first we wash with PBS, after that we add 2 ml...

01 January 2016 5,402 12 View

Does anyone having experience working with Decitabine?

I am working on bladder cancer cells. I am using Decitabine drug for my study. For experiments i dissolved drug in DMSO and after that i aliquoted it and stored it in -80. Every time i used a...

10 October 2014 7,946 5 View

Does anyone have experience working with decitabine drug?

I am working in cancer biology. I want to study effect of demethylating agent on expression of certain genes in a cell line. I have chosen the decitabine drug for treatment of the cell line. Which...

01 January 2014 3,159 9 View

What is the best method for RNA isolation from tissue samples?

I am working on human tissue samples. I am studying expression of some genes. For that I have to make cDNA from RNA which I isolate from tissue samples. We can use various methods like chopping,...

12 December 2013 9,714 12 View

Can any one tell me how to design primer for Methylation specific PCR?

I am new to this field, can any one tell me any specific site regarding this and how to design primer step wise manner.

10 October 2013 7,379 2 View