Can any one tell me about IDH1/2 mutated transfected cell lines, is it available commercially or if any one have?

Saquib Ashraf @Saquib_Ashraf

01 January 2012 1 4K Report

If not then any one can tell me how can i transfect this mutated plasmid (the easiest way).

thank you

Regards

Gui-Rong Li

You may follow this web site http://www.nejm.org/doi/full/10.1056/NEJMoa0808710 to find the publication about it.

Good luck!

GR Li

PS: In case your University is not authorized to access this Journal, the PDF article is attached.

Badges
Science method

More Saquib Ashraf's questions See All

Is there any IDH mutated leukemic cell lines available?

I need IDH1 and IDH2(R140Q & R172K) mutated and wild type transfected/infected leukemic cell lines, can anyone tell me either this is available commercially or if anyone having this cell line?...

06 July 2012 2,200 3 View

How can I decontaminate mycoplasma?

I am using Leukemic cell lines like U937, HL-60 etc for drug treatment after infection/transfection, but unfortunately my cell lines are contaminated with mycoplasma, can any one tell me is this...

06 July 2012 5,879 16 View

How do you prepare and use ascorbic acid in cell treatment?

As we all know ascorbic acid is very unstable in aqueous solution because of its oxidizing behavior, can anyone please tell me if I want to do treatment with ascorbic acid to cell lines then how...

03 April 2012 2,644 14 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

Transfection of A549 using lipofectamine 3000?

Im trying to transfect A549 cells using lipfectamine 3000. im using a 96 well plate with 1microgram plasmid DNA in each well . should i remove the culture media or change it or decrease it befor...

21 July 2024 7,610 2 View

Why there is a absence of colonies on the Abx plate for selection after liposome mediated DNA transfer ?

Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...

16 July 2024 4,698 4 View