Hi sir/madam
I have successively extracted dried leaves of M.oleifera by maceration using hexane,chlorofrom,ethyl acetate, hydroalcohol,water. By physicochemical tests hexane, chloroform, ethylacetate has given positive result for terpenoids. but I did not get any spot on TLC by using butanol:ethylacetate mobile phase mixture. kindly suggest how to optimize the method for identification on TLC.
Please look at the following below links which may help you in your analysis:
https://pdfs.semanticscholar.org/20ca/4b1fded49f130e02278dbf67a0e2f2d0e56e.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113832/pdf/nihms794864.pdf
http://cdn.intechopen.com/pdfs/32738/intech-column_chromatography_for_terpenoids_and_flavonoids.pdf
Thanks
https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2006-959408
Regards
Dear Uma Rajeswari Batchu Mam, I did some similar experiments where I did extraction from non-polar to polar solvents, and subjected the extracts to TLC, after playing around with various solvent systems I found, Hexane:Ethyl Acetate in 6:3 and Hexane:Dichloromethane:Acetone in 5:4:1 and Chlorofom:Methanol in 9:1 system, of all these system Hex:EA gave good separation and CHCL3:MeOH also gave good separation. Please let me know if the system is working fine for you. Best of Luck!!
Dear Saurav Roy sir, I worked with the Hexane:Ethyl Acetate in 6:3 combination but little change in composition (5:4). I got seperation on TLC plate. Thank you for sharing your experience.
Glad to hear that mam, I have came over a master separation system(worked well in so far every extract used), if you are interested, i can share that with you, please message me in research gate, I'll contact you via mail.
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