Uma Rajeswari Batchu

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Questions related from Uma Rajeswari Batchu

How to prevent autolysis of uric acid in borate buffer of pH 8.5?

I have prepared 10mM uric acid in borate buffer of pH 8.5 to study the uricase assay as per previous literature. However, the uric acid exhibited autolysis with borate buffer and tris buffer...

09 February 2023 7,384 0 View

How to overcome the practical problem arised during in vitro assay of XO inhibition?

Dear sir/Mam I have been trying to study the Xanthine oxidase inhibition of 1-ortho methyl chrysophanol isolated from actinobacteria. The formula was (A-B)-(C-D)/A-B X100. Here C is...

24 June 2021 622 0 View

Can any one suggest the best mobile phase composition for seperation of triterpenoids from hexne extract of plant materials?

Hi sir/madam I have successively extracted dried leaves of M.oleifera by maceration using hexane,chlorofrom,ethyl acetate, hydroalcohol,water. By physicochemical tests hexane, chloroform,...

20 June 2019 7,141 6 View

How do seperate triterpenes from Petroleum ether extract of leaves?

Kindly provide the reference articles for seperation of terpenoid fraction from pet ether extract.

23 April 2019 3,951 3 View