I am trying to observe DNA fragmentation in HeLa cell lines, but every time i isolate DNA and run Agarose electrophoresis only thing i can see is smear.
I have used C-TAB method for isolating DNA fragments.
In the image of gel from left
Lane1: DNA ladder (1Kb)
Lane 2: Control untreated
Lane 3: test compound treated
Lane 4: H2O2 treated
please suggest a good and tested method