Can any one suggest me a protocol for isolation of plasmid DNA for staphylococcus aureus?

More Archana Loganathan's questions See All

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Anyone can list the activities based on body schema which will enhance the nonverbal communication of mentally retarded children ?

02 July 2024 9,947 0 View

How to perform electropolymerization on the screen printed carbon electrode?

I want to perform electropolymerization on 3 system carbon electrode. How to cover the reference and counter electrode? I want to make polymer only on working electrode.

23 May 2024 1,681 3 View

Which books should i read to learn the basics of Magnetic Nanoparticles?

I want to learn the Magnetism of nanostructured materials in very deep. Please suggest me some books , if anyone of you have suggestions.

18 May 2024 7,415 1 View

Electro-optical Characteristics Measurement System- LCMS. Is the VT curve means graph between Driving voltage and relative transmittance?

After measuring VT curve using Electro-optical Characteristics Measurement System like LCMS, how to plot graph. What is PD voltage and relative transmitance. Is the VT curve means graph between...

15 May 2024 2,393 0 View

How to calculate yield percentage using HPLC data?

I have depolymerized byproducts and commercialized products. I doubt how to calculate yield percentage using HPLC data. Can anyone give me an answer? It will be helpful in my research career....

29 April 2024 9,448 5 View

How to fix the molarity of 2 analytes to be interacted in Gauss View using DFT ?

I am trying to optimise the molar ratio of 2 analyte (For example A and B) during a reaction. For this purpose I want to optimise there molar ratio. For example A:B = 1:2, 1:3, 1:4, etc. Which...

28 April 2024 5,775 2 View

How to perform TEM imaging of chitosan nanoparticles using cooper coated TEM grids?

Is it possible to get a proper TEM images of chitosan nanoparticles as a film of nanoparticles are formed on the cooper grid?

16 April 2024 368 3 View

How can I develop an enzyme assay for degradation of gluten( low quantity of enzyme available)?

I need to develop an enzyme assay to evaluate the degradation of gluten using proteases. Previously, I conducted tests with finished products, which was straightforward. However, I now have to...

07 April 2024 1,886 3 View

What is the most effective enzyme for breaking down gluten formed when flour is mixed with water?

I've selected protease as the optimal enzyme for eliminating gluten formed from flour. Could you please provide insights on the best enzyme for removing gluten, dosing methods, and how to identify...

24 March 2024 1,618 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View