I have to check the heterochromatin of the dendritic cells by transmission electron microscopy. Therefore I want to know the fixation, dehydration, embedding (in which material) in detail. Kindly suggest.
I know how to extract the effective medium parameters of an electromagnetic metamaterial absorber for normal incidence by Nicolson-Ross-Weir technique. But, how can I extend this to angular...
18 August 2023 296 0 View
Could anyone share a protocol for the isolation of Sarcoplasmic reticulum from mice muscle ?
20 February 2023 4,566 3 View
I am using 1900 mAh pouch cells for the testing. Feel free to ask further details related to the questions
28 December 2022 7,801 0 View
An acceptor-acceptor polymeric electrode material is showing lesser charging time in same current density at all potential ranges than discharging time. As I know this may be due to some...
25 November 2022 2,535 1 View
Are there any reports on biomagnification of micro-plastic as reported for pesticides? Accumulative movements of microplastics in higher strata through the food web and its consequential adverse...
13 August 2021 618 4 View
Hello research community, While going through EIS of aged cells, I found that the cells EIS data is different every time. For example, Cell A kept at storage for one day for testing at 50% SOC to...
03 August 2021 2,902 2 View
I trying to do frequency calculation after optimization calculations using mixed basis set for lanthanides SDD and 6-311++G(d,p) for other atoms with %mem=2GB. The error shows: Not enough memory...
21 October 2020 4,548 0 View
I am trying to do frequency calculation after optimization calculations using mixed basis set for lanthanides SDD and 6-311++G(d,p) for other atoms with %mem=2GB. The error shows: Not enough...
21 October 2020 9,846 3 View
I have two Li-ion cells one charged and other discharged, same chemistry anodes and same manufacturer. I have Nyuist plots ontained by single sweeps in Bode 100 and also Frequency plots. How do I...
06 September 2020 1,320 1 View
I am looking for a method or component that helps maintaining MPP while charging battery from solar power without using buck-boost converter (may be a cheaper device can be used) Can anyone give...
01 June 2020 10,219 5 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View