I have differentially expressed circular RNA candidates. Are there any data, database or bioinformatical pipeline to extract circular RNAs can potentially be translated into proteins? Thank you!
I also hope to get an answer
We are try to HARDOX weld with FSW ? Is it possible ? I'm curious your opinion can you share me?
26 March 2024 9,438 3 View
Hi all, We seek an efficient electroporation protocol for patient-derived preadipocytes and SGBS cells to perform co-transfection for CRISPR/Cas9. They are pretty sensitive and hard to transfect....
12 February 2024 4,856 0 View
How can I calculate the SOMO value to be able to perform SOMO-HOMO calculations? When I do the HOMO LUMO calculations of the structure I'm working on (spin doublet), I see two values due to the...
12 April 2023 6,654 2 View
Hello! I am trying to start a new experiment using 68Ga and 177Lu labeled peptides on LnCaP and PC-3 cells to find out the binding, internalization and uptake values. First of all, the terms are...
21 November 2022 8,405 0 View
Hi everyone, I have a proteomics data and want to compare male-female mice protein expression levels. I need to find differentially expressed proteins between two group. Student's T test just...
05 October 2022 9,681 2 View
I am planning to use an overexpression strategy to create a Halo-tagged fusion RBP protein, then aimed to perform RIP strategy by using Magne® HaloTag® Beads to reveal hypothesized RNA: Protein...
14 April 2022 695 0 View
I tried to perform RNA immunoprecipitation assay to reveal RNA partners of the desired protein. Can I store cell extract not the lysate- after cross-linking (1% FA, 10 min RT)- at -80 degrees? Best.
14 April 2022 321 1 View
PBS poly(butylene succinate) electrospinning process conditions.
03 March 2022 1,720 8 View
We are working on a study where we compare pre and post-workshop answers from a group of subjects. Subjects answered a question before the workshop and answered the same question after the...
17 January 2021 3,056 6 View
Hi there; I am measurin DPPH antioxidant activity by Trolox standard and need an urgent help if possible since I am so confused with the calc. In extraction process; I mixed 5 gr of my sample...
19 January 2019 380 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View