Can we joining HARDOX to HARDOX by Friction Stir Welding ?

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Could anyone recommend an efficient electroporation protocol for primary preadipocyte cells or SGBS cell line?

Hi all, We seek an efficient electroporation protocol for patient-derived preadipocytes and SGBS cells to perform co-transfection for CRISPR/Cas9. They are pretty sensitive and hard to transfect....

12 February 2024 4,856 0 View

How to calculate SOMO value in Gaussian program?

How can I calculate the SOMO value to be able to perform SOMO-HOMO calculations? When I do the HOMO LUMO calculations of the structure I'm working on (spin doublet), I see two values due to the...

12 April 2023 6,654 2 View

Anyone has a cell binding, internalization and/or uptake protocol using radiopharmaceuticals?

Hello! I am trying to start a new experiment using 68Ga and 177Lu labeled peptides on LnCaP and PC-3 cells to find out the binding, internalization and uptake values. First of all, the terms are...

21 November 2022 8,405 0 View

Differentially expressed proteins?

Hi everyone, I have a proteomics data and want to compare male-female mice protein expression levels. I need to find differentially expressed proteins between two group. Student's T test just...

05 October 2022 9,681 2 View

Anyone perform RIP by using Magne® HaloTag® Beads?

I am planning to use an overexpression strategy to create a Halo-tagged fusion RBP protein, then aimed to perform RIP strategy by using Magne® HaloTag® Beads to reveal hypothesized RNA: Protein...

14 April 2022 695 0 View

Can I store cell extract, which crosslinked with formaldehyde, at -80 degrees before lysis ?

I tried to perform RNA immunoprecipitation assay to reveal RNA partners of the desired protein. Can I store cell extract not the lysate- after cross-linking (1% FA, 10 min RT)- at -80 degrees? Best.

14 April 2022 321 1 View

Have you ever experienced PBS poly(butylene succinate) electrospinning?

PBS poly(butylene succinate) electrospinning process conditions.

03 March 2022 1,720 8 View

What test(s) do you recommend for non-parametric binary data?

We are working on a study where we compare pre and post-workshop answers from a group of subjects. Subjects answered a question before the workshop and answered the same question after the...

17 January 2021 3,056 6 View

Can any one suggest a way to analysis of translational potential of candidate circular RNAs?

I have differentially expressed circular RNA candidates. Are there any data, database or bioinformatical pipeline to extract circular RNAs can potentially be translated into proteins? Thank you!

10 April 2020 8,628 1 View

How can I calculate %inhibition trolox equivalent in DPPH assay?

Hi there; I am measurin DPPH antioxidant activity by Trolox standard and need an urgent help if possible since I am so confused with the calc. In extraction process; I mixed 5 gr of my sample...

19 January 2019 380 3 View

How should operational parameters be selected when developing a pore-free laser welding technology?

There are three operating parameters - the laser power, the diameter of the focus spot and the scanning speed. It is necessary to obtain a seam of a given depth without pores. What parameter...

27 July 2024 7,168 2 View

What are the important scholarly articles related to 'Use of Likert Scale in social science research' ?

The Likert scale is a commonly used measurement tool in social science research to assess people's attitudes, opinions, or perceptions. The social science students still have difficulty selecting...

25 July 2024 7,535 1 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Masonry Interaction Module ABAQUS?

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16 July 2024 5,047 0 View

Can an average household DIY spot welder be used for pouch cell electrode tab?

Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?

10 July 2024 8,763 0 View

Designing Inclusive City. What's your opinion regarding my group's campaign below?

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07 July 2024 368 0 View

Can light itself have a temporal property? I mean, does light capable to dilate time if conditions require it?

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03 July 2024 4,332 1 View

How to calculate corrosion rate from potentiodynamic polarization test data for dissimilar welds?

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30 June 2024 5,943 2 View

What is the difference between opportunity recognition in entrepreneurship literature and sensing in dynamic capabilities theory?

While I do have some opinions on how to address this question based on my reading as student, I would like to know the opinions of more accomplished scholars.

28 June 2024 802 3 View

My material is AlSi10Mg made by Selective laser melting. Why i am facing difficulty in EBSD?

1. Which phase should I select during EBSD? 2. Attached image is the polished surface but in your opinion what is on the surface of sample? it is oxide or colloidal? 3. Diamond polishing by paste...

27 June 2024 5,248 2 View

Vipin Sharma

It is hard, so ceramic tools and preheating the plate can make it weldable.

Shovgi Asadov

This technology is mainly used in welding non-ferrous metals with low melting temperature. Your opinion is also interesting.

Bilge Kaan Tuysuz

Thank you both of them for your answers. I think preheating will be working but we can't do because our system doesnt give permission.

Our tool material is WC with%10Co. Our first try was failed. We will try again but we need to more information because every material is so expensive we dont have a any supporter.

Our main project is Inconel&Inconel, Inconel&Hardox, Hardox&Hardox FSW.