Bilge Yaylak

6 Questions 1 Answers 0 Followers

Questions related from Bilge Yaylak

Could anyone recommend an efficient electroporation protocol for primary preadipocyte cells or SGBS cell line?

Hi all, We seek an efficient electroporation protocol for patient-derived preadipocytes and SGBS cells to perform co-transfection for CRISPR/Cas9. They are pretty sensitive and hard to transfect....

13 February 2024 4,897 0 View

Can I store cell extract, which crosslinked with formaldehyde, at -80 degrees before lysis ?

I tried to perform RNA immunoprecipitation assay to reveal RNA partners of the desired protein. Can I store cell extract not the lysate- after cross-linking (1% FA, 10 min RT)- at -80 degrees? Best.

15 April 2022 362 1 View

Anyone perform RIP by using Magne® HaloTag® Beads?

I am planning to use an overexpression strategy to create a Halo-tagged fusion RBP protein, then aimed to perform RIP strategy by using Magne® HaloTag® Beads to reveal hypothesized RNA: Protein...

15 April 2022 736 0 View

Can any one suggest a way to analysis of translational potential of candidate circular RNAs?

I have differentially expressed circular RNA candidates. Are there any data, database or bioinformatical pipeline to extract circular RNAs can potentially be translated into proteins? Thank you!

11 April 2020 8,663 1 View

How to prepare highly efficient (10^8) TOP10 competent cells?

Hello, I used CaCl2 Ionue method to prepare TOP10 competent cells two times, but obtained 10^6 efficiency in each case unlike DH5a that i prepared before which was very efficient. The OD of my...

11 April 2020 9,539 0 View

How Circular RNAs are enriched by RNAse R treatment ?

I am going to perform circ RNA enrichment by RNAse R treatment and I have searched for the appropriate protocol but unfortunately did not find any detail. Most of them used different time...

30 October 2017 8,565 3 View