What is best solution for optical metamaterial design calculation: HFSS, COMSOL, CST?

Dear all, Now I'm selecting FEM for design of metamaterials on NIR~VIS frequencies, and keeping 3 software: ANSYS HFSS, COMSOL with wave optics module, and CST STUDIO SUITE. Could you give me...

09 February 2021 9,517 2 View

How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

29 July 2020 3,784 2 View

What is the significance of polydispersity of nanoparticles?

What information does polydispersity of nanoparticles, calculated in percentage from the ratio of standard deviation and mean particle size give?

18 September 2019 8,538 3 View

Containment strategies for antibiotic resistance?

How can the spread of resistant genes in microbes be contained #antibioticresistance #antibiotics #microbes #bacteria

20 August 2019 3,006 4 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

20 December 2017 9,821 5 View

How to measure (~50 kV) very high voltage?

I need to measure high voltage in transmission electron microscope (in electron gun)

31 July 2016 520 3 View

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

12 November 2015 6,372 3 View

How can we define an "unethical intelligent agent" in a cooperative multi agent environment?

08 April 2015 5,406 14 View

Does anyone know of an automatic Approach-Avoidance Task (AAT) for chocolate (or similar)?

I am conducting research into the prediction of chocolate consumption by implicit processes, and in addition to measuring implicit attitude, I want to measure automatic approach-avoidance tendency...

27 November 2013 4,270 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View