Can any one share with information on or the source of ELISA for measuring Antisnake immunoglobulins in Human?

More Godwin Imade's questions See All

Which stain is best when it comes to identifying rumen flukes?

20 June 2024 5,591 0 View

Possible collaboration on Veterinary, probiotics, Zoonosis and One health research ??

My name is Godwin Dogbey, a veterinarian in Ghana. This year, I am planning a number of questionnaire-based research works and would like to form a team of hardworking young researchers from...

29 January 2024 4,904 2 View

How to get a map of Nigeria showing coco yam distribution?

I need a map showing where coco yams are planted in Nigeria

05 December 2023 9,856 1 View

Snowball sampling technique?

In using a snow ball sampling technique, is sample size calculation required?

01 October 2023 929 0 View

Pre-print? what is a Preprint?

If my paper is published as a preprint, does it mean that the paper has been rejected?

18 September 2023 7,078 1 View

Are you willing to collaborate with me on a study on awareness of the veterinary profession amongst the public.?

I am trying to assess public perception of the veterinary profession and issues. I have prepared the questionnaires already and would need collaborators( who will double as coauthors) to look...

21 August 2023 9,707 2 View

Will it be appropriate to access the knowledge of human pharmacists on zoonosis?

16 August 2023 7,373 5 View

Please how much will it cost me to purchase primers for infectious laryngotracheitis?

Please how much will it cost me to purchase primers for infectious laryngotracheitis? and where can i purchase these primers?

11 August 2023 6,046 2 View

Looking for a collaborator to look over my questionnaire before I roll out a study on veterinary professionals' knowledge of zoonosis?

Looking for a collaborator to look over my questionnaire before I roll out a study on veterinary professionals' knowledge of zoonosis. Contact me if you have the expertise and can assist.

11 August 2023 3,513 5 View

How do you feel when your article is accepted, especially in high impact factor journal?

Recently, the rejection rate is becoming alarming. The way people react when their papers are rejected seems not to be same when it happens in the opposite.

02 July 2023 1,967 12 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View