How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

29 July 2020 3,784 2 View

What is the significance of polydispersity of nanoparticles?

What information does polydispersity of nanoparticles, calculated in percentage from the ratio of standard deviation and mean particle size give?

18 September 2019 8,538 3 View

Containment strategies for antibiotic resistance?

How can the spread of resistant genes in microbes be contained #antibioticresistance #antibiotics #microbes #bacteria

20 August 2019 3,006 4 View

Can any one share with information on or the source of ELISA for measuring Antisnake immunoglobulins in Human?

Is There a commercial ELISA Kits for measuring Antisnake venom? Please share source and cost

10 September 2018 3,342 2 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

20 December 2017 9,821 5 View

How to measure (~50 kV) very high voltage?

I need to measure high voltage in transmission electron microscope (in electron gun)

31 July 2016 520 3 View

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

12 November 2015 6,372 3 View

Does anyone know of an automatic Approach-Avoidance Task (AAT) for chocolate (or similar)?

I am conducting research into the prediction of chocolate consumption by implicit processes, and in addition to measuring implicit attitude, I want to measure automatic approach-avoidance tendency...

27 November 2013 4,270 5 View

Containment strategies for antibiotic resistance

Treatment of diseases arising from AMR has largely proved ineffective time and again. So shouldn't containment strategies be outlined to regulate spread of resistant genes?

01 January 1970 704 5 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View