How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

29 July 2020 3,784 2 View

What is the significance of polydispersity of nanoparticles?

What information does polydispersity of nanoparticles, calculated in percentage from the ratio of standard deviation and mean particle size give?

18 September 2019 8,538 3 View

Containment strategies for antibiotic resistance?

How can the spread of resistant genes in microbes be contained #antibioticresistance #antibiotics #microbes #bacteria

20 August 2019 3,006 4 View

Can any one share with information on or the source of ELISA for measuring Antisnake immunoglobulins in Human?

Is There a commercial ELISA Kits for measuring Antisnake venom? Please share source and cost

10 September 2018 3,342 2 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

20 December 2017 9,821 5 View

How to measure (~50 kV) very high voltage?

I need to measure high voltage in transmission electron microscope (in electron gun)

31 July 2016 520 3 View

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

12 November 2015 6,372 3 View

Does anyone know of an automatic Approach-Avoidance Task (AAT) for chocolate (or similar)?

I am conducting research into the prediction of chocolate consumption by implicit processes, and in addition to measuring implicit attitude, I want to measure automatic approach-avoidance tendency...

27 November 2013 4,270 5 View

Containment strategies for antibiotic resistance

Treatment of diseases arising from AMR has largely proved ineffective time and again. So shouldn't containment strategies be outlined to regulate spread of resistant genes?

01 January 1970 704 5 View

Should scientists also be cautious about vaccine-induced immune pressure driving mutagenesis and advantageous escape viral variants?

It is excellent news about the COVID-19 vaccination programme and the vaccination trials where both humoral and cellular immune responses to challenge, after vaccination are observed. These...

02 August 2020 1,738 4 View

What is the risk of entomopathogenic diseases acquiring the ability to succesfully infect humans?

I broadly understand the scale at which Zoonoses develop, but would be deeply curious to understand if there is a similar risk of entomopathogenic diseases jumping the biological border to...

27 July 2020 2,239 3 View

Is there any plant disease(s) that can infect and/or cause illness in human?

Just like Zoonosis, is there a disease(s) that can be passed from plants to humans? Can plants carry harmful germs, such as bacteria, fungi, and viruses that can infect humans and cause diseases?

06 July 2019 3,291 12 View

What steps do you follow to standardize an ELISA with 4PL?

Dear, please, could you share me, protocols on how I can standardize ELISA using 4PL, I am going to thank you. I am a novice in this and I would like to learn, since in the literature they do not...

03 April 2018 659 0 View

Any recommendations for some websites give information about updated emerging infectious diseases like Health map or WHO ????

01 February 2017 7,645 13 View

I'm trying to do LAMP but with degenerated primers for relapsing fever borrelia group. Does anyone have experience with it?

I know that regarding the concentration of primers I've have to used 4 to 5x more and I've done that. However only one species amplifies. Any suggestions? Thanks in advance! :)

23 November 2015 3,745 1 View

Can anyone help with analysis using TreeMap or Dendroscope to construct Tanglegrams?

I need some assistance to address an issue concerning host switch events.

09 October 2015 3,998 1 View

Which one of the following sentences is correct?

06 July 2015 6,105 0 View

Is anybody interested in collaboration studies with camel brucellosis?

17 February 2015 1,381 3 View

Is anybody interested in collaboration studies with camel brucellosis?

01 February 2015 9,336 4 View