I would like to monitor ester hydrolysis with GC. can any one help me by pointing out if there are any reactions that can be done so. Thank you.
Dear Sir. Concerning your issue about the reactions which can be monitored by GC. A fast GC-FID method for monitoring acidic and basic catalytic transesterification reactions in vegetable oils to methyl ester biodiesel preparation was carried out. The model reaction was conducted in the presence of internal standard (hexadecane or acetophenone). Foranalysis, 1-5 μL of sample was taken from the reaction mixture and was diluted with a suitable solvent (e.g. hexane or ethyl acetate). The diluted solution was injected into a GC-MS using an autosampler. Each GC reading is the average of three runs. Typically three reactions under the same conditions were conducted in parallel. For more details you can see the following below links which may help you in your analysis:
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000300352 http://www.rsc.org/suppdata/c5/cc/c5cc05306d/c5cc05306d1.pdf http://wiki.aapg.org/Gas_chromatography/mass_spectrometry_(GC/MS):_procedures http://www.nature.com/nprot/journal/v8/n12/fig_tab/nprot.2013.144_F7.html
Thanks
GC is separation and determination tech. Products of reactions can be monitored which help you conclude the nature of rxn.
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