Fluorescence quantum yield is the ratio of the number of photons emitted by the fluorophore to the number absorbed. The possible values are between 0 and 1.
Avinash Mithilesh Singh could you send me this paper I dont have access to the article
What is fluorescent quantum yield and how do you calculate it?. Available from: https://www.researchgate.net/post/What_is_fluorescent_quantum_yield_and_how_do_you_calculate_it [accessed Mar 20, 2015].
Relative and absolute determination of fluorescence quantum yields of transparent samples
Mr. Khan please find the attachment. I am attaching another file from horiba which has a step by step description of QY calculation. Cheers
available from :https://www.researchgate.net/post/What_is_fluorescent_quantum_yield_and_how_do_you_calculate_it?amp%3Bch=reg&%3BloginT=JtwJiXmDyyXuDr1Zj7eb8e4rm4Dq6RrQxqYmCJKQ4hg*&%3Bpli=1#550d6092d4c118c9468b4649
Quantum yield is the efficiency of the molecules that how much light is being absorbed and emitted..its ranges between 0 to 1..in order to calculate quantum yield, you must have the reading of standard compound, whose absorption intensity somewhat in good agreement with your molecule at the same concentration what you have chosen for absorption and emission of your test sample also while taking the spectra should have the same slit width, somewhat same excitation wavelength.