05 May 2017 2 3K Report

I want to use PEI to adhere bacterial cells on a glass slide for microscopic observation

More Prithwiraj Kirtania's questions See All
What are the causes for fabricated S11 and simulated S11 parameter for a CPW- Fed monopole antenna?

I have simulated a CPW fed monopole antenna in ANSYS HFSS. I have also fabricated it. But results are not as good as the HFSS simulation. I renormalized the port. I fabricated it twice. But there...

20 March 2020 8,235 6 View

General recommendation of hydrogel in soil. How is that effective?

General recommendation of hydrogel in soil is nearly 8-15 kg/ha. Which means, in only 15 cm furrow slice, only 6 ppm of hydrogel conc. in soil. I want to know that how that minute amount be...

17 October 2018 4,821 7 View

Is the discharge from the first drip and the discharge from the last drip is always equal?

With the increasing length of lateral, due to more frictional forces and the more openings encountered in the path, pressure head difference is evident in drip laterals.

01 September 2018 5,270 0 View

Is there is any way to randomize the drip irrigation experiments?

Its a general trend in India that we are not able to use randomization in experiments on drip irrigation due to the fact that a same lateral can not be used for different treatments. Rather we...

01 September 2018 4,870 2 View

Does any one answer if Tyloxapol gives a smooth de clumped culture of M tuberculosis?

I am trying to get a mycobacterial suspension culture which should be totally declumped. I am using 0.05% tween but failed to get desired smoothness with Mtb. Can any body give me some inputs...

12 July 2017 7,656 4 View

Is it possible to increase pulse productivity through Nitrogen fertilization? (Only yield maximization is desired rather than resource saving.)

Pulses are known to fix atmospheric nitrogen through bacterial symbiosis. But only a single molecule of fixed nitrogen consumes about 16 molecules of ATP and the carbohydrate provided to the...

01 January 1970 9,973 19 View

Relevance of physical weed management

Now a days, less and less soil disturbances are desired. At the same time we are conscious about the environmental harm caused by herbicides. So, in this context can physical weed management holds...

01 January 1970 8,878 7 View

Similar questions and discussions
Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close