Can any one help me to find applications of plant crude polysaccharide isolated from malvaceae family ?

More Muhammed Haneef Koolikkunnu's questions See All

Which method is best to induce liver damage for study hepatoprotective activity of plant extracts on mice/rat?

Literature says that Paracetamol and Carbon tetrachloride (CCl4) induced damages can be used to find out the antioxidant potential/ hepatoprotective activity. Which is best method among these two?

04 May 2018 5,522 2 View

What are the methods to find out anti inflammatory acitivity of plant extracts?

kindly help me in finding out standard method for both in vitro and in vivo antiinflammatory activity of plant extracts.

02 March 2016 1,658 8 View

Which mask is best against inhalation of organic solvents?

I am using different organic solvents in my lab. can any one suggest me best masks to prevent inhalation of these toxic solvants.

11 December 2015 431 10 View

Can anybody help me to remove free sugars from crude plant extracts?

I need to remove free sugars from plant crude extracts (metahanol). is there any method to achieve this?

08 September 2015 7,192 10 View

How do I remove mucilage from plant crude extracts?

Hi, can any body tell how to separate mucilage from crude methanolic extracts of bark and leaves of Malvaceae member.? Is it possible to use the separated non mucilage part and mucilage part for...

07 August 2015 3,269 9 View

Can anyone explain the logic behind acidifying crude extract for fractionation?

Please explain the reason behind acidifying extract before fractionation? will it make any difference in extraction, with or with out acidifying?

07 August 2015 1,288 4 View

Can anyone suggest which solvent combination can be used to separate compounds from crude methanolic plant extract by TLC method ?

I am trying to separate and isolate all individual compounds from different plant extracts (extracted with methanol, ethyl acetate, chloroform, water). I tried chloroform-methane, hexane- ethyl...

05 June 2015 9,897 22 View

Which solvent is better to dissolve different solvent extracts for antimicrobial assay (Disc diffussion method) ?

Hi, I am doing antibacterial activity of plant extracts (Methanol, chloroform, ethyl acetate and water.). And I am using DMSO as solvent to dissolve extracts, but some extracts are not dissolving...

02 March 2015 8,931 12 View

Which is the best method to separate and characterize compounds from Crude plant extracts with polar and non polar solvents?

Which is the best method to separate and characterize compounds from Crude plant extracts (Polar and non polar solvents )? Please suggest different techniques.

11 December 2014 3,502 7 View

How to prepare Pollen grain sample for SEM (Scanning Electron Microscopy)?

I have dried anthers of one particular plant, and I need to take SEM images of pollengrains for surface morphology. What procedure should I follow? Please help me.

10 November 2014 1,929 14 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

What is the best method to conserve the medicinal plant facing rapid decline in population ?

I have identified specific medicinal plant facing rapid decline in population due to population pressure, overutilization, unsustainable harvesting and limited distribution. What can be the best...

15 July 2024 6,599 2 View