I am doing flow cytometry for the genome size determination of my plant samples. But the histograms obtaining every time is quite confusing.Is any one have an experience in plant flow cytometry data analysis?
I have my plant small RNA data from illumina. Done all quality filtration steps and conserved miRNA predictions. Now I have to predict novel miRNAs. In order to align, reference genome is not...
01 February 2018 3,741 8 View
Do any one have an experience in using plant cyc database? I have done the blastx of my assembled transcriptome with plantcyc.fasta file. The outfmt6 file generated shown some IDs only and no...
05 June 2016 5,048 6 View
We identified conserved and novel miRNA from my plant samples (non model plant). The targets were predicted through psRNA target using Arabidopsis as the background reference but targets were not...
02 March 2016 6,733 3 View
I ran MIRA with 1.2 GB (after pre processing) of my plant ion proton transcriptome data in a 120 GB RAM system. We are working through a rented server. Currently it is running and generated...
02 March 2016 1,587 4 View
I have conserved and novel miRNAs of my plant samples and done the target prediction. Can any one please suggest me how could or any computational tools for analyzing the miRNA regulating pathways?
31 December 2015 9,742 9 View
I have my transcriptome data of plant samples. Since the reference genome of my plant or its closest species is not available i have to go for denovo transcriptome assembly. Before assembly do i...
09 October 2015 7,222 7 View
I am currently doing the NGS data analysis of miRNAs from my plant samples and after pre processing the reads were aligned against plant miRNAs from miRBase. In the proceeding step all paper...
08 September 2015 8,778 12 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View
In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?
13 July 2024 6,296 5 View