Dear Shaaya,

The HL60 cell line is the cell line you are looking for! It can be differentiated towards neutrophil like cells by different kinds of stimuli. Just use the link:

https://www.google.at/?gfe_rd=cr&ei=WA7iVLaFHMuH8Qet2YDYCg&gws_rd=ssl#q=hl60+neutrophil+differentiation

Kind regards,

Robert

I have used the HL60 cell line. They need some care when they are differentiating but they respond similar to neutrophils and have comparable granular composition, I think

Much thanks to all. The links given by Robert actually contains lots of valuable papers/information, and of course, much appreciated.

I am currently differentiating PLB-985 cells using ATRA and DMF and still interested in recent (2014/2015) published work involving differentiation of cell line into neutrophil-like. 

This paper has referenced a few literature where they show HL-60 differentiation: http://www.ncbi.nlm.nih.gov/pubmed/25653484

Pubmed has information on PLB-985 differentiation using DMF as well as DMSO. http://www.ncbi.nlm.nih.gov/pubmed/?term=plb+985+differentiation

Fantastic Avinash! that's in deed very relevant to my search.

Thank you Robert and Avinash for your valuable answers.

With kind regards.

Shaaya

Hello Shaaya,

I worked in the past with the cell line MPRO clone 2.1 which can be differentiated into mature neutrophils (http://www.ncbi.nlm.nih.gov/pubmed/9808058)

The cell line HOXB8-ER is also a great model for studying neutrophils (http://www.ncbi.nlm.nih.gov/pubmed/16554834)

Both cell lines take about 6 days to be differentiatiated into neutrophils and, in my opinion, are more neutrophil-like than HL60 derived cells.