Can aequorin being stably transfected in cell line?

10 September 2019 1 2K Report

We are trying to transfected the aequorin plasmid into neurons, how can aequorin being stably transfected in cell line?

Yes! Aequorin can be stably transfected in cell lines. The apo-enzyme apoaequorin is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin, the active form of the enzyme. Aequorin stable cell lines have traditionally been generated by transfection and co-expression of aequorin with a specific target of interest. See the attached. Article A novel rapid and reproducible flow cytometric method for op...

https://patents.google.com/patent/US5714666

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