Can acetone precipitation remove SDS from protein solution?

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Steingrimur Stefansson

Hi Sudipa, I'm afraid these solvents will not work in removing SDS from your protein.

I recommend using detergent removal columns from Pierce:

https://tools.thermofisher.com/content/sfs/manuals/MAN0011676_Pierce_Detergent_Remov_SpinColumn_UG.pdf

Christian Q. Scheckhuber

The suggestion by Steingrimur is probably the way to go. However, there are also low-cost alternatives. Please check the paper in the attachment if you want to know more.

Regards, Christian

Article Removal of dodecyl sulfate from protein solution

Ahmed Neama

I suggested to use detergent removal columns from Pierce:

Carlos Araújo Queiroz

Cf.: A. Alam, "Agent for protein precipitation, a method of protein precipitation, a method of protein assay using protein precipitation agent, and a kit for protein assay", US Pat. 6,875,617 B2; Apr. 5, 2005.