"Peptide matches not assigned to protein hits" what does this mean?

I have three different tryptically digested samples. But the PMF for all seemed to be same. Tough the intensity of few peaks are very high, MS and MS MS of the abundant peaks in the MASCOT Search...

06 July 2017 5,867 0 View

Can acetone precipitation remove SDS from protein solution?

Is SDS soluble in solvents like acetone , TCA or ethanol?

03 April 2017 6,637 4 View

Can we run carbohydrates (not glycoproteins) in SDS PAGE? If not, why?

SDS PAGE is used to separate proteins acc to mol weight. Can we use this system for separating oligosaccharides?

01 February 2017 807 10 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Italicization in molecule name: N-acetyl-p-benzoquinoneimine (NAPQI)?

Is there a convention for correct italicization of abbreviated parts of chemical molecule names? For...

13 January 2021 7,396 3 View

Was there any pesticide disasters other than Bhopal and Kasaragood tragedy around the globe?

Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...

11 January 2021 3,790 7 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View