We want to perform a human RNA extraction from cell culture for an RNA-seq, but we have a viral RNA extraction kit (Quick-RNA™ Viral Kit-Zymo research) available. Therefore, we want to know if any methodological issues can interfere with the results if we use the viral kit.
It is generally not recommended to use a viral RNA extraction kit for the isolation of human RNA, as the kit is specifically designed for the isolation of viral RNA and may not efficiently extract human RNA. Additionally, the reagents and protocols used in the kit may not be optimized for the isolation of human RNA and may lead to poor quality or quantity of RNA. It is recommended to use a kit specifically designed for the isolation of human RNA, such as the Quick-RNA™ MicroPrep Kit-Zymo Research.
On one occasion, we mistakenly used a viral kit for human RNA, and the yield we obtained was low.
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