Accidentally we have added EDTA 50mM to an AAV-virus lysate when we were trying to stop the benzonase reaction. We inmediately diluted the lysate to a final concentration of 17mM because we had no option. Any advice or suggetion will be appreciated!. I have read that some people are using 10mM to inhibit benzonase reaction successfully but still I would like to have more opinions. Thanks!
Victor U. Weiss
Hi! We experienced for human Rhinovirus (non enveloped, single stranded RNA virus) that intermediates of virus uncoating are stabilized by increased conc of Mg2+. So, yes, I would suggest that EDTA plays a role , however, it depends on your further experiments if it's useful or not...
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