José Diego Pignataro

3 Questions 16 Answers 0 Followers

Questions related from José Diego Pignataro

How much could mycoplasma affect an AAV production?

I was keeping HEK 293T cells for an AAV production, although my last mycoplasma test was positive. I already discard the cells in order to avoid more contamination in our culture room, but I would...

30 January 2015 8,021 7 View

Can a high concentrations of EDTA affect the virus stability during the purification step?

Accidentally we have added EDTA 50mM to an AAV-virus lysate when we were trying to stop the benzonase reaction. We inmediately diluted the lysate to a final concentration of 17mM because we had no...

08 July 2014 3,309 1 View

Could polybrene affect lentivirus infection efficiency in HEK 293T cells?

I have performed an infection in 293T cells with a purified and concentrated LV stock (for GFP expression). One day after plate 293T cells in a p100, I prepared the medium (DMEM 10%FBS+Pen/Strep)...

20 February 2014 4,399 7 View