Can a Drosophila chromosome lost its FRT sites without a FLP?

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Yuan-Yeu Yau

Do you know the sequences flanking the two FRT sites? If so, you can PCR them and sequence them to find out.

Hammed Badmos

Thanks very much Dr Yau. I will do just that. I have done something preliminary with G418 food but they are not resistant. Do you have insight into why FRT might be lost without FLP so that I can prevent future occurrences? Regards.