Hi! I have conjugated a CD34-FITC antibody to 80 nm gold particles. When I stain the cells with the conjugate, I get high rates of dead cells when measuring with trypan blue staining. The ratio of dead cells in the sample ranges between 30%-70% depending on the dilution (the more concentrated the antibody solution, the higher the death rate). Has anyone experienced similar events? How did you solve the issue?
Thank you in advance!
Steingrimur Stefansson
Hi Daniela Lascu . It could be that there is something in the Au-Ab particle prep that is killing the cells. You could try gel filtration or dialysis to exchange the particle solution with a physiological buffer.
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