Daniela Lascu

3 Questions 2 Answers 0 Followers

Questions related from Daniela Lascu

Trypan Blue overestimates the viability of cryopreserved PBMC?

Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...

11 July 2024 613 1 View

Can 80nm gold bound antibodies be toxic for cells?

Hi! I have conjugated a CD34-FITC antibody to 80 nm gold particles. When I stain the cells with the conjugate, I get high rates of dead cells when measuring with trypan blue staining. The ratio of...

01 July 2021 8,656 1 View

Is the band in non-reducing SDS Page of IgG antibody an impurity or degradation?

Hi! I have purchased a CD34-FITC (IgG) antibody from a company and in order to conjugate it with a gold nanoparticle, I had to purify it and remove all the stabilizing proteins. I used a BSA...

21 June 2021 7,703 6 View