Vibhu Mishra

3 Questions 1 Answers 0 Followers

Questions related from Vibhu Mishra

Can 8 molar urea alone is capable of breaking the disulphide bonds in proteins during cell lysate preparation?

Actually i need to purify the his tagged proteins and check for the formation of disulphide bonds,for which I am using TCA and HU buffer without dtt or merceptoethanol

05 March 2019 7,680 2 View

What will happen to my digested vector if digestion is done for longer than 8 hours?

I am using EcoRI HF and Xho I with cut smart buffer? It's 100 ul reaction with 1.25 of each enzyme and around 1 ug of vector?

10 October 2018 1,092 1 View

Can RNA interfere with the digestion of plasmid dna?

Will it be utmost necessary to remove RNA from plasmid mini prep for further digestion?

01 January 1970 2,218 5 View