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Questions related from Sebastian Fiamma
Hi all, for the past 6 weeks i have tried and failed to transfect JurKat cells, first i tried with pNL1.2 vector as it is required for the assay im working on, later i switched to pIRES-eGFP...
15 October 2024 9,835 2 View
As the question already implies, i made a mistake during restriction enzyme digestion and added the 6x loading dye to the DNA insert fot checking on the gel. 10ul was added to the gel for checking...
15 February 2023 6,096 2 View
We are working on vector isolation in the lab, the second time we did the isolation we got a concentration of 50ng/ul which is to lower then desired. The protocol followed siad to use a 1.5 ml...
10 February 2023 4,145 2 View
