I need help in calculating FPU using CMC as cellulose alternative where i missed the dilution step which is adding 20 ml distilled water as mentioned in CARBOXYMETHYL CELLULASE ASSAY FOR ENDO-13-1, 4-GLUCANASE (ref. 8)
where i got 0.5 ml crude enzyme resulted in 1.92 mg glucose
what i am missing here?