The following data are obtained in an enzyme catalyzed reaction: [S]x10^4(moldm^-3): 2.5, 5.0, 10.0, 15.0.
Then rox10^6(moldm^-3min^-1) : 2.2, 3.8, 5.9, 7.1.
Assuming Michaelis-Menten Kinetics, calculate:
1. rmax
2. Km
3. K2
Using the Lineweaver- Burk and Eadie method.
Given [E]o = 4.0x10^-6 mol/dm^3
1. rmax- maximum rate
2. Km- Michaelis Menten constant
3. [S]- concentration of substrate
4. ro- initial rate
Check the image for clarity.
There is little data (experimental points) for obtaining reliable values of parameters of the M-M equation.
Hi there,
I guess you have been formed to MM theory. If so the work consists just in the basic use of the MM equation to determine catalytic parameters. To help you just a bit LB plot is linear if the system obeys to the MM equation. 1/Vm is y for x=0 and -1/Km is x for y=0. Be careful with the units you use! Then k2 calculation is based on the fact that Vm=k2*E0. Here again careful with the units which have to be consistent...
Regardless of whether we use nonlinear regression or one of the many graphical methods, e.g. L-B, E-H or Merino, nothing will change because the number of points is too small to determine reliables values of two parameters. Additional kinetic tests should be performed for rate limiting substrate concentrations below 2.5 and above 15.
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