17 September 2018 8 615 Report

Thank you in advance for your help. My myotubes are plated in 12 well plates coated in Matrigel in 10% FBS DMEM and allowed to reach confluency before being switched out to 1% FBS DMEM (day 1 of differentiation). Media is changed every other day and the myotubes look great around day 5. They cover the entire plate and are straight/long.

Around day 6-7 they begin to start acting weird and I don't know why. The myotubes become branched and fuse together in different directions, and ultimately they die. The way they fuse is almost a mesh-like pattern.

Has anyone else had this problem? I've checked the incubator for correct temp and CO2. It's not contamination, either. Thanks!

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