After read a paper where they use glycerol (but do not explain de reason ) I am thinking in use:

-10% Glycerol with de G-250, BN Buffer Page and the other stuffs

Also I use to>

Use only Dark Blue for coomasie staining

Use Frist Dark B until 1/4 of front has ran and then Light Blue for Silver staining.

And in the same paper the change to native buffer after de Dark Blue, so skip de Light blue.

I have some problems with hight hydrophobic proteins in my BN-Page gels, so...do you think that these changes could be nice for me?