Dear jadoon, I am also a Ph.D student in the same field. For a pure culture of SRB it is mandatory to supply vitamins and micronutrients, and that is the purpose of adding Yeast extract. You also simply can add NH4Cl, vitamin solution and trace elemental solution (Widdel and Pfennig 1982), you will gate same or better result. Hope it will be helpful for u.
I am highly obliged to both of you guys for your kind concern. Dear Tobias Goris, you are absolutely right that generally we use yeast for un-defined medium. So, I have the same situation that my medium is defined one and I can not use yeast. In my experiment, I want to calculate how much lactate (as a source of carbon) has been utilized by Desulfotmaculum australicum. So for this purpose I used to calculate the initial concentration of lactate added in medium, then calculate the total organic carbon (TOC) after filtering medium by 0.2 micrometer pore size filter to remove the cells. Now this medium is left with dissolved carbon which is mostly the remaining lactate. However, on other hand I have used some growth factor and some vitamins and trace elements but failed to get growth. Now I am using the vitamins and trace elements suggested by Bidus Kanti Das, but I am afraid that if I couldn't get growth with this combination then it would be really bad luck for me. Tobias Goris if I failed to get growth this time (as I am using new combination of vitamins and trace elements), what should I do? I have read in literature that we add yeast as growth promoter in case of Desulfotomaculum genera, can I compromise with addition of yeast but lower the quantity of it in medium?
Dear Bidus Kanti Das I have cultured my strain in your suggested vitamins and trace elements and NH4Cl is already present in my prepared medium. I also hope that it will work.
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