Does anybody know what company (preferably in US) has FBS with low endotoxin (
See attached file for the COA of the FBS we use in our lab.
Dear colleagues!If dsRNA has 2 bulky modifications on its 3'-ends (for example protein on one end and a nanoparticle on another), may it create problems for Dicer?Maybe somebody met something in...
26 October 2014 3,137 3 View
I am conducting archival research on the issues that parents have communicated about with their children in letters they mailed to their children. I am interested in things that gratified parents...
02 June 2014 3,491 3 View
I am trying to talk about reasons for popularity of crime movies in France during World War 1 and want to link it to war fears.
16 May 2014 1,424 4 View
Does anyone know of research being done on elastography, done on the testes for general tissue properties and disease states rather than focal lesions? Does anyone know of a good reference on upto...
16 October 2013 8,698 1 View
I need to obtain monoclonals for it.
25 April 2013 7,999 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View