Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Post Hoc for 2-Way Repeated Measures ANOVA?

I have a 2 x 3 repeated measures design with significant interaction, and I used LSD as a post hoc for the interaction but it does not adjustment the alpha. Which correction would be the most...

05 March 2020 4,301 3 View

Blue native gel fluorescence?

Hi, Did anyone visualize fluorescent bands for fluorescent tagged proteins on a blue native gel? Do you destain the gel after the run? If anyone has a working protocol I would appreciate...

11 December 2018 7,336 5 View

Site directed mutagenesis of 5 consecutive sites in one reaction?

Hi, I am willing to mutate 5 specific consecutive amino acids to Alanine in a certain insert using site-directed mutagenesis in one PCR reaction only. I'm scanning a certain area of my protein to...

31 December 2017 3,704 1 View

Crowding and false FRET problem?

I'm using the Operetta system to measure the FRET signal between two proteins in the Golgi (tags are YFP and mCherry). The majority of the signal comes from protein crowding and not from real...

07 August 2017 9,106 5 View

I need identification key to species of genus Nymphaea in the world?

I found new wild species of Nymphaea in Egypt. I registered it in our herbarium CAIM. The material of the new species was compared with herbaria specimens of Nymphaea in CAIM, and CAI. In...

13 April 2017 2,765 3 View

Applying load in ANSYS, sometimes not working?

02 June 2016 2,456 4 View

What is the best way to measure the flow rate of superheated steam in a small tube?

24 March 2015 9,039 8 View

Why is the intercept term (or constant coefficient) not present in the panel FMOLS estimation regression ?

e.g: this is the regressed regression through the method of Cointegration (panel FMOLS- Fully Modified OLS), but there were no intercept term in the regression equation given by E-views...

30 December 2014 3,482 3 View

Are there computational biology algorithms/applications that can be used for in silico high-throughput screenings?

If not, do you think is it possible to simulate this in order to predict which genes might be real hits, of course before the real in vitro experiments?

20 November 2013 3,041 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View