I am blocking some microwells with PBS + BSA 1.0 % and then sensitizing them with LPS. Please let me know what range of absorbance values I should expect for a microwell without sensitizing with LPS, but with the blocking buffer used.
Adam B Shapiro
You haven't said much about the experiment, but I'll guess you are doing an ELISA-type measurement. The question is, what is the background absorbance in the negative control (no LPS) blocked well. It's impossible to know for sure in your experiment, but in my experience a negative control well had a low absorbance of less than 0.1 in the case of an HRP-conjugate using TMB for detection.
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