Confirmatory correlation analyses, after Anova?

Dear everybody, I conducted a series of ANOVAs (and MANCOVAs) to verify the presence of differences between two groups for a series of variables. A reviwer commented that " the relationship they...

02 November 2020 4,982 6 View

How to model in Arena Rockwell that all entities must clear their tails at the end of the simulation?

I am modeling the flow of visitors during a working day in a museum. The main problem is that I don't know how to model in Arena Rockwell the fact that all entities must clear the queue at the end...

20 February 2020 9,457 1 View

LC-MS: same compound, 2 RT?

Hi, I have an issues with the detection of a standard on LC-MS. I run MGDG standard (prepared in methanol, LC-MS grade) on HILIC BEH Amide column; the run is 15 minutes long, using a gradient...

16 June 2019 4,355 4 View

How would you morphological gloss truncated words in spontaneous speech in an Italian dialect?

I have a data set of spontaneous speech in Romanesco (Italian dialect spoken in Rome), which I need to gloss morphologically. These are the turns in question (but there are other similar...

20 February 2019 7,567 3 View

Chloroform resistant tips in lipid analysis?

Hi, I'm wondering if someone is using carbon filtered pipette tips for lipid analysis. At the moment I extract lipids from bacterial cultures using methanol:chloroform and I use Hamilton syringe...

22 January 2019 2,829 1 View

Can predictors of a Dependent variable be considered as control variables if those are not the main focus of a study?

Hello, I am carrying out SEM analysis with a model including two Independent variable (A,B) one mediator (C) and two dependent variable (D,E). For reasons of statistical control and incremental...

06 March 2018 8,275 3 View

Do you have an exact reference to know if I have to restart from passage number zero when I thaw cells?

Concerning numeration cells passage, there are two school of thought. One said that we have to restart from zero and one said that we have to continuing the numeration (e.g. freeze at #4, maintain...

19 July 2016 7,135 2 View

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I am trying to figure out whether a CO2 (GHG) emission reduction goal of -60% by 2030 is feasible for Switzerland and am happy to have any inputs, reading advice etc. Thanks!

24 June 2014 8,401 3 View

What are the best cell lines to use as control for Casr gene expression using real-time PCR?

We test Casr expression in human stem cells with real-time PCR (Taqman probes, Life technologies), but only using a large amount of DNA (about 250ng) we observed ct at 32-34 cycles. We would...

04 February 2014 3,073 1 View

Do you know a method for phytochelatin extraction by HPLC?

I would like information on a methods for phytochelatins determination with HPLC and without the use of the additional step of derivatization and without MS, if possible.

15 December 2013 8,573 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View